![]() ![]() On the whole, caseinase production was profoundly highest (15.01 %) while the least prevalent virulence characteristic observed among tested beach Enterococci was haemolysis of rabbit blood (3.65 %). A total of 160 isolates were also tested for virulence characteristics. The species classification of encountered Enterococci resistance to vancomycin was highest among Enterococcus spp. Chi-square analysis showed no significant association between responses to tested antibiotics and the species allocation or source of isolation of all tested Enterococci. High-level resistance to kanamycin was higher among Enterococcus faecalis and Enterococcus faecium than Enterococcus spp. However, high-level resistance to kanamycin (2,000 μg/mL) was observed in 14.2 % of tested isolates, the highest proportions observed being among beach sand isolates. None of the encountered isolates were resistant to high levels of gentamicin (500 μg/mL). We report the first study on the occurrence of high-level aminoglycoside-resistant (HLAR) Enterococci in coastal bathing waters and beach sand in Malaysia. Strain G6 bacterium showed greatest gelatinase activity towards porcine gelatine which can be potentially used for porcine gelatine identification. Application of partially purified gelatinase onto porcine, bovine and fish capsules substituted into the gelatin medium (GM), respectively resulted in 11.86 a ± 0.2 U/ml, 5.39 b ± 2.1 U/ml and 0.36 c ± 0.2 U/ml of enzyme activity, respectively. The molecular weight of gelatinase of Strain G6 was 123.35 kDa. The partially purified gelatinase of Strain G6 showed significant different (p<0.05) in porcine gelatinase activity of 9.12 a ± 2.6 U/ml followed by bovine (5.43 b ± 0.8 U/ ml) and fish gelatine (0.14 c ± 0.7 U/ml). Gelatinase from the bacterium has been partially purified using deposition of ammonium sulphate and tube dialysis. Meanwhile, by using Biolog GEN III, the bacterium strain was identified as Lysinibacillus sphaericus analysis at 42% of similarity. Strain G6 using the partial sequence of 16S rDNA analysis with 95% of similarity. The bacterium was identified as Lysinibacillus spp. The Strain G6 colony was chosen due to its high ability to hydrolyse porcine gelatine when it was grown on medium containing porcine gelatine by forming a clear zone. (Strain G6) for hydrolysing porcine gelatine. This study was conducted to identify and characterize gelatinase bacterium of Lysinibacillus spp. ![]()
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